Late complications with gallbladder conduit biliary reconstruction after liver transplantation.

537 determined by correlating the recipient's serum reactivity against the T and B lymphocyte and monocyte populations as well as the use of HLA-specific monoclonal blocking antibodies. Thus, a procedure is now available for better prediction of graft survival in the recipient. The usefulness of this new procedure is particularly important in patients with high levels of sensi-tization. Investigators have noted that finding a serologically compatible kidney for these individuals is very difficult (I5). Since the modified flow cytometric procedure can make the distinction between deleterious and irrelevant antibodies, some potential recipients may still be considered for transplantation with an available kidney despite a positive B cell crossmatch result. In conjunction with the CDC assay, we intend to use this modified flow cytometric cross match technique to study antibodies reactive with donor leukocytes in order to improve the success rate of renal transplantation. In summary, we have described a modified flow cytometric crossmatch technique that utilizes donor peripheral blood leu-kocytes. The use of leukocytes provides a wider spectrum of antigens and has the potential to distinguish between antibod-ies that are detrimental and those that have no effect on graft survival. The procedure can also be performed in a shorter period of time than lymphocytotoxicity and previous flow cy-tometry crossmatch procedures. Three cases were described in which the modified crossmatch procedure was useful in characterizing the antibody responsible for the positive B cell CDC crossmatch. The application of this procedure in renal trans-plantation allows for the use of extended phases of crossmatch testing, thus enhancing the probability of graft survival. At the same time, some potential allograft recipients are not excluded from consideration for transplantation solely on the basis of a positive crossmatch result. cross match and glomerular rejection in renal transplantation.eral lymphocytes express more HLA antigens than T peripheral lymphocytes.cute rejection of kidney allografts associated with preexisting humoral antibodies against donor cells. Lancet 1966; 2: 662. analysis: a high technology crossmatch technique facilitating transplantation. Influence of pre-transplant antibodies on early renal allograft rejection. The preferred techniques for biliary tract reconstruction with liver transplantation are duct-to-duct anastomosis over a T-tube stent or anastomosis of the graft common duct to a defunctionalized Roux limb of jejunum (1-3). A more complex but occasionally useful procedure is the gallbladder conduit operation, which was recommended by Waddell and Grover (4) for use in liver transplantation and adapted by CaIne (5) for this purpose. In our …